anti human pedf capture antibody  (R&D Systems)

Journal:

Article Title: The alternative pathway is required, but not alone sufficient, for retinal pathology in mouse laser-induced choroidal neovascularization

doi: 10.1016/j.molimm.2010.12.016

Figure Lengend Snippet: Quantitative RT-PCR was performed on RPE-choroid samples removed 7 days post laser photocoagulation from control (C57BL/6), CFB−/− (no-AP) and C1q−/− MBL−/− (AP-only) mice. Quantitative values were obtained by cycle number (Ct value), determining the difference between the mean experimental (e.g., VEGF) and control (β-actin) ΔCt values. With the exception of C3 mRNA expression, mRNA levels for all the angiogenesis (VEGF and PEDF) and complement markers (C9) were normalized in CFB−/− (no-AP) and C1q−/− MBL−/− (AP-only) mice when compared to the C57BL/6 control. No consistent trends were obtained in the membrane-bound complement inhibitors CD55 and CD659 or the soluble inhibitor CFH. Tissues from 3 animals per group were evaluated.

Article Snippet: To measure production of VEGF and PEDF by the RPE-choroid, eyecups were solubilized in CellLytic MT (mammalian tissue lysis/extraction reagent; Sigma) and centrifuged at 20,000 g for 5 min. Microplates were coated with either the anti-mouse VEGF (Antigenix America, Inc.) ( Rohrer et al., 2009 ) or the anti-human PEDF capture antibody (R&D Systems, Minneapolis, MN) ( Ma et al., 2009 ), and 100 μL of the tissue extract was added.

Techniques: Quantitative RT-PCR, Control, Expressing, Membrane

Journal:

Article Title: The alternative pathway is required, but not alone sufficient, for retinal pathology in mouse laser-induced choroidal neovascularization

doi: 10.1016/j.molimm.2010.12.016

Figure Lengend Snippet: Quantitative ELISA assays were performed on RPE-choroid samples removed 7 days post laser photocoagulation from control (C57BL/6), CFB−/− (no-AP) and C1q−/− MBL−/− (AP-only) mice. Quantitative values were obtained by using a standard curve of purified mouse protein (VEGF) or by determining the fold difference between experimental (e.g., CFB−/− and control C57BL/6) values (PEDF). Protein levels for VEGF (A) and PEDF (B) followed the mRNA levels; both were normalized in CFB−/− (no-AP) and C1q−/− MBL−/− (AP-only) mice when compared to the C57BL/6 control. Tissues from 3 animals per group were evaluated.

Article Snippet: To measure production of VEGF and PEDF by the RPE-choroid, eyecups were solubilized in CellLytic MT (mammalian tissue lysis/extraction reagent; Sigma) and centrifuged at 20,000 g for 5 min. Microplates were coated with either the anti-mouse VEGF (Antigenix America, Inc.) ( Rohrer et al., 2009 ) or the anti-human PEDF capture antibody (R&D Systems, Minneapolis, MN) ( Ma et al., 2009 ), and 100 μL of the tissue extract was added.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Purification